Description: This course provides detailed lectures on Biotechnology, covering concepts like microscopy, autoradiography, etc.
Curriculum
- 1 Section
- 41 Lessons
- 10 Weeks
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- Analytical Technologies in Biotechnology41
- 2.1Basic concepts in microscopy 1
- 2.2Basic concepts in microscopy 2
- 2.3Dark-field and phase contrast microscopy
- 2.4Differential interference contrast and polarization
- 2.5Fluorescence and confocal microscopy
- 2.6Transmission electron microscopy
- 2.7Transmission electron microscopy cont. and scanning electron microscopy
- 2.8Basic concepts 1
- 2.9Basic concepts 2
- 2.10GM counting and Scintillation counting
- 2.11Scintillation counting continued
- 2.12Autoradiography and RIA
- 2.13Safety aspects and applications
- 2.14Introduction and Basic concepts in chromatography 1
- 2.15Basic concepts in chromatography 2
- 2.16Low-pressure liquid chromatography (LPLC) and high performance liquid chromatography (HPLC)
- 2.17Ion-exchange chromatography
- 2.18Gel-filtration chromatography
- 2.19Affinity chromatography
- 2.20Gas-liquid chromatography
- 2.21Basic concepts in electrophoresis
- 2.22Horizontal and vertical gel electrophoresis
- 2.23Native gel electrophoresis and SDS-PAGE
- 2.24Isoelectric focusing (IEF), 2-D gel electrophoresis and protein detection methods
- 2.25Electrophoresis of nucleic acids
- 2.26Immunoelectrophoresis and capillary electrophoresis
- 2.27Introduction and basic concepts 1
- 2.28Basic concepts-2
- 2.29Types of centrifuges and analytical ultracentrifugation method
- 2.30Separation methods in preparative ultracentrifuges
- 2.31Types of rotors
- 2.32Types of rotors cont. and care of rotors
- 2.33Introduction and basic concepts
- 2.34UV-Visible spectroscopy
- 2.35Infrared and fluorescence spectroscopy
- 2.36Circular dichroism (CD) spectroscopy
- 2.37Nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography
- 2.38Atomic spectroscopy and mass spectrometry
- 2.39Polymerase chain reaction(PCR)
- 2.40DNA sequencing methods
- 2.41Enzyme linked immunosorbent assay (ELISA)
